1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Function of lectins in the efficiency of ferritin nanoparticle vaccines
Paper ID : 1027-ICB10
Authors:
zeinab masoomi1, مریم عظیم زاده ایرانی *2, سید مسعود حسینی2
1velenjak, shahid shahryari square, shahid beheshti university
2دانشکده علوم و زیست فناوری
Abstract:
Introduction
Viral infections constitute a serious public health and social problem worldwide, especially during the recent COVID-19 pandemic caused by the betacoronavirus SARS-CoV-2.[1] Display of antigens on ferritin generally elicits a more robust neutralizing antibody response against the target pathogen as compared to immunization with the antigen alone.[2] The ferritin-RBD nanoparticle vaccine induced not only a persistent RBD specific antibody response but also long-term protective memory. [3] Because the ferritin nanoparticle vaccine uses glycosylated RBD (receptor binding domain) in its structure, it may be recognized and gets inefficient by human lectins. Lectins are glycan-binding proteins, present ubiquitously in all forms of life.[4] In this study, molecular docking to examine the lectin binding site on the ferritin nanoparticle vaccine protein used.
Material & Methods
The lectin protein structure was obtained from the protein data bank (PDB id: 5b1w). haddock 2.4 server was used to dock the lectin and ferritin nanoparticle vaccine complex together and biomolecular visualization of the complex was carried out by pymol.
Results
The structure of the full length human lectin was obtained in complex with RBD-ferritin nanoparticle. Lectin (residues:51-64) binds close to the RBD-ferritin interface (residues: 41-49) and Haddock score (3.7 +/- 12.1) and RMSD value (10.9 Å) of the molecular docking showed that this connection has low stability and it does not interfere with ferritin and RBD binding. The results suggesting that efficiency of ferritin nanoparticle vaccine may Not be affected by human lectins.
Conclusions
Binding of human lectin to the RBD-ferritin nanoparticle shows that although the binding site of the lectin is near to the RBD and ferritin interface it does not inhibit the ferritin-RBD binding.
Keywords:
Lectin, RBD-ferritin, Nanoparticle vaccine, Molecular docking.
Status : Paper Accepted (Poster Presentation)