1st International and 10th National Iranian Conference on Bioinformatics

The Effect of a Single Nucleotide Polymorphism on a miRNA Function in the Papillary Thyroid Carcinoma (PTC)

Paper ID : 1045-ICB10

Authors:

Fatemeh - Skandari, Pegah Javid, Mansoureh Azadeh *

Zist Fanavari Novin biotechnology institute

Abstract:

Papillary Thyroid carcinoma (PTC) is the most common form of well-differentiated thyroid cancer, and the most common form of thyroid cancer to result from radiation exposure. PTC has shown strong heritability and no predisposing mutations in the germ-line. In the current study, we have researched the effect of common T/G polymorphism (rs1357866392) as an SNP on the miR-34a-3p sequence and its binding to the mRNA sequence of MET proto-oncogene, receptor tyrosine kinase gene (MET) (ENSG00000105976.14) in PTC through bioinformatics. The MET gene encodes a member of the tyrosine kinase receptor family of proteins and the MET proto-oncogene product. For this purpose, GEO datasets showed the top 250 differentially expressed genes. The David database was used to cluster the gens and it revealed the involved genes in transcriptional misregulation pathway in cancer including MET gene. The miRNASNP-v3 database showed the relationship between studied miRNA and SNP. The results showed that binding to the 3’UTR region of MET, the miR-34a-3p blocks gene’s expression, and acts as a tumour suppressor in PTC. The occurrence of rs1357866392 (T/G on chr1:9151709) in the seed match of miR-34a-3p (chr1:9151708-9151714) and MET (chr7:116796124-116798386), made the binding to be lost. It makes the gene not to be under the control of miRNA anymore and upregulation will occur. Thus, our data suggest that rs1357866392 is a disease-associated SNP since as a common polymorphism in miR-34a-3p affects the regulation of the MET gene and result in the genetic predisposition to PTC. Its role in the tumorigenesis through somatic mutation Preliminary evidence suggests that these effects are mediated through target genes which expressions are affected by the SNP status.

Keywords:

PTC, MET gene, miR-34a-3p, rs1357866392

Status : Paper Accepted (Poster Presentation)