1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
The role of miR-429 in regulation the activity of colorectal carcinoma cells via autophagy using system biology approaches
Paper ID : 1047-ICB10
Authors:
Seyed Taleb Houseini *1, Mansoureh Azadeh2, Arash Sattari3, Farkhondeh Nemati1, Roya BishehKolaei1
1Department of Biology, Faculty of Basic Sciences, Islamic Azad University, QaemshahrBranch, Mazandaran, Iran
2Zist Fanavari Novin biotechnology institute
3Department of Medical laboratory sciences, faculty of medical sciences, Gorgan Branch, Islamic Azad University, Gorgan , Iran
Abstract:
Background: Colorectal cancer (CRC) is among the most frequent cancers (3rd) and is one of the major causes of cancer-related deaths (4th) worldwide [1,2]. Autophagy is a highly evolutionary conserved housekeeping process that relies on the degradation of cellular components (e.g., organelles, protein aggregates) in autophagosomes. Altered activity in this pathway (compared to normal conditions) is associated with numerous human diseases, including cancer [3,4]. In this study, we investigated the role of miR-429 on the CRC cells in autophagy.

Material and Methods: In this study, For extracting miRNAs target genes, miRTareBase, miRWalk, miRDB, DIANA databases were selected for predicted purposes. Genes related to CRC cell activation were extracted from Coremine database and then the common genes were selected. Cytoscape software were used to graph the protein-protein interaction network. Finally, the signaling pathways and functional analysis of the final genes were examined using the Enrichr database.

Results: The results of miR-429 targets showed that out of 183 genes obtained in Coremine database and 1252 genes in the mentioned databases, 6 genes including ZEB1, TFA2PA, FBXW7, WASF3, CRKL and RND3 in the process of CRC cell activation are common. Finally the protein-protein interaction network was drawn and examined.

Conclusion: By examining the target genes of miR-429 in the process of activating CRC cells and mapping the protein-protein interaction network of these genes and analyzing the signaling and functional pathways, it was found that the mechanism of autophagy plays an effective role in this process. The results of this study can help to clarify the mechanism of activation of CRC cells.
Keywords:
Colorectal Carcinoma, miR-429, autophagy, system biology
Status : Paper Accepted (Poster Presentation)