1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Computational Analysis of ARID1A and its correlated miRNAs Involved in Colon cancer
Paper ID : 1048-ICB10
Authors:
Seyed Taleb Houseini *1, Mansoureh Azadeh2, Arash Sattari3, Farkhondeh Nemati1, Roya BishehKolaei1
1Department of Biology, Faculty of Basic Sciences, Islamic Azad University, QaemshahrBranch, Mazandaran, Iran
2Zist Fanavari Novin biotechnology institute
3Department of Medical laboratory sciences, faculty of medical sciences, Gorgan Branch, Islamic Azad University, Gorgan , Iran
Abstract:
Background: Colorectal cancer (CRC) is one of the most common gastrointestinal malignant cancers and is a leading cause of cancer-related mortality across the globe [1]. miRNAs are a new family of endogenous non-coding RNAs, almost 20-24 nucleotides in long that arrange the expression of various genes involved in normal development as well as human disease including cancer[2]. ARID1A is frequently deleted in multiple human tumors. It is located on chromosome 1p36.11, a region that is commonly deleted in various cancer types and suspected to contain tumor suppressor genes[3-5]. In this study, we aim to focus on discovering the crosslink between ARID1A and its related miRNAs as a key oncogene in colon cancer.

Methods: The interaction between ARID1A and its target miRNA was found by implementing miRTargetLink database. Overall, 98 miRNA was found to be highly associated with ARID1A expression. Among the predicted miRNAs, three of them were nominated for their strong correlation with ARID1A in colon cancer. The candidate miRNAs were further examined for KEGG enrichment pathway analysis by using Diana miRpath V.2 algorithm.

Results: The analyzed data obtained from miRTargetLink databases showed that hsa-miR-31-5p, hsa-miR-101-3p and hsa-miR-1-3p are significantly correlated to ARID1A. Furthermore, by using Diana miRpath algorithm, we demonstrated that these miRNAs play important roles in the cell cycle.

Conclusion: Taking into account the low level of expression of hsa-miR-31-5p, hsa-miR-101-3p and hsa-miR-1-3p in colon cancer and their direct interaction with ARID1A, it can be observed that these miRNAs can severs as potent regulators of signaling pathways involved in colon cancer progression.
Keywords:
Computational Analysis, ARID1A, microRNAs, Colon cancer
Status : Paper Accepted (Poster Presentation)