1st International and 10th National Iranian Conference on Bioinformatics
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An immunoinformatics approach for the design of a multi-epitope vaccine targeting lukD of Staphylococcus aureus
Paper ID : 1057-ICB10
Authors:
Parisa Khodabandeh Shahraki *1, Mokhtar Nosrati2
1Isfahan Endocrine and Metabolism Research Center,Isfahan university of medical science,Isfahan,Iran
2Department of Biotechnology, Faculty of Advanced Science and Technologies, University of Isfahan, Isfahan, Iran
Abstract:
Background: S. aureus is the pathogen most frequently isolated from diabetes foot ulcer. These days, antibiotic-resident S.aureus is an active debate in science and clinical world. Today we can harness immunoinformatics tools with reverse vaccinology principles to design a multi epitope vaccine. In this study we aim to design a vaccine against lukD and lukE, which are two of most frequent virulence factor of S. aureus in diabetes foot ulcer.
Methods: The present study used immuno-informatics and computational approaches to identify both T-cell and linear B-cell epitopes of the lukD virolance factor of S. aureus. The three-dimensional structure of the final vaccine protein and conformational B cell epitopes was predicted with online servers.
Results: Among linear B-cell epitopes, "NITPKREKKVDD", "GDINISNGLSGGLN", and "NYKQESYRTTIDRKTNHK" were identified as highly antigenic. Among T-cell epitopes, "FQILTFNFI" (MHC class-I peptide) and "TSTYEVDWQNILLKL", "LNIFQILTFNFIKDK", "KLNIFQILTFNFIKD", "NDKLNIFQILTFNFI", "NQNTVTFTSTYEVDW", "LGKSSVASSIALLLL" (MHC class-II peptide) displayed the highest population score, and was immensely antigenic and conserved. All selected epitopes was non-allengen. We put these epitopes together with KK linker. Human Beta-defensin 1 and PADRE sequence were used as adjuvant. In vaccine tree dimensional structure, four conformational B-cell epitope were detected.
Conclusion: The present study identified several epitopes of the lukD virulence factor of S. aureus that are highly antigenic and conserved.
Keywords:
S.aureus, Staphylococcus, T-cell epitopes, B-cell epitopes, bioinformatics, Computational approaches, Vaccine design
Status : Paper Accepted (Poster Presentation)