1st International and 10th National Iranian Conference on Bioinformatics

Home Accepted Papers

Bioinformatics Analysis of the Expression of Key Genes Involved in Biosynthesis Pathway of Carvacrol of White Savory Essential Oil Under Drought Stress

Paper ID : 1066-ICB10

Authors:

fatemeh davari shalamzari *, لیلا نژاد صادقی, خسرو مهدی خانلو

دانشکده کشاورزی، دانشگاه شهید چمران اهواز

Abstract:

White savory (Satureja mutica Fisch. & CAMey) is a member of the mint family that is extended in the eastern and western of Iran. This plant has antioxidant and antimicrobial properties. In this study, White Savory was planted in two conditions of drought control and treatment at 30% of field capacity in the Faculty of Agriculture, Shahid Chamran University. Using the KEGG database, the biosynthetic pathway and genes involved in the production of this pathway were determined. The active ingredient in White Savory is Carvacrol, in which three key genes including: Geranyl geranyl de phosphate synthase, Limonene synthase, Limonene hydroxylase are involved in the biosynthesis pathway of Carvacrol. Utilizing bioinformatics and real-time PCR, the expression of genes effective in biosynthesis of active substance and biochemical pathways was largely determined. Due to the lack of white savory genomic information, the gene sequences of plants of this family were examined. After predicting the sequence of these three genes, suitable primers were designed. In this study, the intracellular location of proteins was determined to be all three cytoplasmic genes. The properties and characteristics of each gene were also examined. Investigation of physical and chemical properties of proteins that are unstable proteins in all three genes, signal prediction of potential peptides in proteins and prediction of spatial structure of proteins, which includes structural evaluation and estimation of structural model quality through bioinformatics detection took. Bioinformatics study revealed that all three genes do not have peptide signal sequences. The quality of the structural model was examined through QMEANDisCo Local and QMEAN Z-Scores. All genes were found to be highly similar to the mentioned structural model. According to the authors of this study, bioinformatics study of genes at the protein level will create more awareness and facilitate future research.

Keywords:

White savory, Carvacrol, Biosynthetic pathway

Status : Paper Accepted (Poster Presentation)