1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Bioinformatics evaluation of hsa-miR-4496 related to a single nucleotide polymorphism (rs1031305330) of GSTP1 gene in gastric cancer
Paper ID : 1089-ICB10
Authors:
sajede naghiyan fesharaki *1, sajjad sisakhtnezhad2, Mansoureh Azadeh3
1Zist Fanavari Novin biotechnology institute,Isfahan,iran
2Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
3Zist Fanavari Novin biotechnology institute,esfahan,iran
Abstract:
Introduction
Gastric cancer remains one of the most common and deadly cancers worldwide [1]. Helicobacter pylori infection is the major risk factor associated with the development of gastric cancer (GC). The transition from normal mucosa to non-atrophic gastritis, triggered primarily by H. pylori infection, initiates precancerous lesions which may then progress to atrophic gastritis and intestinal metaplasia [2]. GSTP1 gene is located position on choromosome11q13.2. Different studies indicated that this gene is associated with gastric cancer. Based on accomplished studies, expression of this gene may be a prognostic indicator for several type of cancer [3]. Single nucleotide polymorphisms (SNPs) are consisting genetic marker related to many of the genetic disease [4]. microRNAs are counted as bio- markers with regulation and control of genes expression at level of mRNA[5]. Given the importance of SNPs and microRNAs as cancer biomarkers that influence the expression of genes, this study aimed to investigate association between hsa-miR-4496 and a single nucleotide ( rs1031305330) of GSTP1 gene in patients suffering from gastric cancer.
Methods
In the present study, databases such as NCBI, miRNASNP-V3, miRWalk, PhenomiR, miRBase and David were used for in silico data analyses.
Results
Our bioinformatics analysis indicated that rs1031305330 is associated with the conversion of C to T allele in GSTP1 gene, which may affect the performance and binding of miRNAs in this region. In this regard, results of this study also revealed that hsa-miR-4496 can bind to the 3'-UTR transcript of the GSTP1 gene.
Conclusion
Altogether, this study identifies rs1031305330 and hsa-miR-4496 as important biomarkers that are associated with GSTP1 gene and gastric cancer. Also, it suggests that the formation of the T allele in rs1031305330 affects the binding of hsa-miR-4496 to the GSTP1 gene.
Keywords:
Single nucleotide polymorphism, rs1031305330, GSTP1 gene, hsa-miR-4496, Gastric cancer .
Status : Paper Accepted (Poster Presentation)