1st International and 10th National Iranian Conference on Bioinformatics

EDC-Protein network formation analysis in genetic response of human epithelial cells to SteA

Paper ID : 1097-ICB10

Authors:

Nazila Eslami *

Abstract:

Salmonella enterica serovar Typhimurium is a gram-negative facultative anaerobic enteric pathogen in humans and animals, and a leading cause of gastroenteritis[1]. The Salmonella pathogenicity island-1(SPI-1) type III secretion system (T3SS) is critical for invasion of host cells via the trigger mechanism by deploying a macropiocytosis-related process in enterocytes and the SPI-2 of the T3SS is responsible for the zipper mechanism and intracellular survival of Salmonella Typhimurium [2,3]. These systems translocate proteins called effectors into eukaryotic host cell. Effectors interfere with certain host signal transduction pathways to allow the internalization of pathogens and their survival and proliferation inside vacuoles. SteA is one of the few Salmonella effectors that are substrates of both T3SSs. Nothing is known about the function of this protein inside the host cells [4].
We scheduled a study to evaluate SteA gene expression in patients with Salmonella-induced gastroenteritis and natural specimens, and obtained effective endocrine disrupting chemicals (EDCs). Then, the protein-protein interaction network was constructed using the STRING database and analyzed using Cytoscape using differentially expressed genes (DEGs) with adjusted p value of less than 0.05. Subsequently, the network produced a PPI module. The genes in the module were then analyzed for GO and pathway enrichment. For module genes, EDC gene interactions were collected and reconstructed as a single EDC gene network.
In Salmonellosis, 324 putative EDCs were discovered to influence gene regulation. The 3 genes TGFB1, CCND1 and LUM were genes that were affected by EDCs. However, these results need to be experimentally confirmed to suggest improved prevention.

Keywords:

Salmonella enterica;Type III secretion system;SteA;MicroarrayHuman epithelial cell; Salmonella pathogenicity island

Status : Paper Accepted (Poster Presentation)