1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Genome-wide identification and characterization of the metal tolerance protein (MTP) gene family in Eucalyptus grandis
Paper ID : 1114-ICB10
Authors:
Zahra Shirazi *1, Fatemeh Khakdan2
1بخش تحقیقات زیست فناوری ، موسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی
2گروه زیست شناسی، پردیس فرزانگان، دانشگاه سمنان
Abstract:
Metal tolerance proteins (MTPs) play an important role in the transport of metals at the cellular, tissue and whole plant levels [1]. Considering the limited information on the MTP gene family in Eucalyptus plants which have phytoremediation properties and their different roles, this study aimed to identify the members, protein characteristics, simple sequence repeats (SSR) markers, miRNAs targets and gene expression analysis of this gene family by bioinformatics tools. The twenty MTP gene members were identified in the genome of Eucalyptus. The phylogenetic tree displayed that MTPs were categorized into three groups (Mn-MTP, Zn/Fe-MTP, and Zn-MTP) and seven sub-groups [2] and were predicted to be localized in vacuoles [3]. EqMTP proteins were predicted to encode polypeptides of 315-884 amino acids with molecular weights ranging from 36.07 to 97.32 kDa. Fifty-seven SSRs were identified in 18 MTP sequences and most of the genes had a single SSR. Identification of the simple sequence repeats (SSRs) marker of this family was found to be helpful in selecting the associated marker. Prediction of MTPs-targeted miRNAs, illustratd 14 EqMTP genes was targeted for 36 miRNAs. All the genes were suppressed by at least more than one miRNA, except for EqMTP9.1. EqMTP12 and EqMTP8.2 were suppressed by eight and seven miRNAs, respectively. Many metabolic pathways and activities related to plant growth and development, signal transduction, and reaction to stresses (including heavy metals) can be arranged by miRNAs [4]. EqMTP11 gene expression was increced in various developmental stages and biotic stresses in different Eucalyptus species. The EqMTP11 gene was proposed as a good candidate for genetic engineering and breeding programs in order to introduce a metal-tolerant cultivar.
Keywords:
Cation diffusion facilitator; Eucalyptus; Heavy metals; Phytoremediation; Metal tolerance protein
Status : Paper Accepted (Poster Presentation)