1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Identification and characterization of raffinose biosynthesis pathway genes in Vitis vinifera
Paper ID : 1115-ICB10
Authors:
Zahra Shirazi *
بخش تحقیقات زیست فناوری، موسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی
Abstract:
Grape (Vitis vinifera L.) is one of the most important fruit crops that various adverse abiotic stresses negatively affect its growth and yield [1]. Raffinose is the classe of soluble carbohydrates that biosynthesis of its starts with activity of galactinol synthase (GolS) enzyme [2]. In the next step, raffinose is made by raffinose synthase (RfS) activity. More than one isoform of GolS and RfS are found in different plant species that are expressed in various organs and responds to diverse environmental conditions [3]. In the present investigation, we carried out a whole genome association study to identify the RfS and GolS gene families, protein characteristics, conserved motives, cis- element in prometor, gene structure and chromosomal location and duplication in grape by bioinformatics tools. Based on protein sequences of GolS and RfS found in A. thaliana; 15 VvGolS and 10 VvRfS were identified in grapes genome which clustered in five and six groups, respectively. The peptide length of RfS and VvGolS members was predicted from 346 to 792 and 162 to 590 amino acid residues. Subcellular localization of all VvRfS members’ family was predicted in cytoplasmic, and most of the VvGolS members were also cytoplasmic. VvRfS had at least three motives of raffinose synthase or seed imbibition protein Sip1domain. The VvGolS family had contained most similar gene structure compared to VvRfS family. The VvGolS and VvRfS genes families were divided into ten linkage groups and four segmental duplication and tandem groups. The existence of four main cis-acting elements (ABRE, LTR, HSE and ARE) indicated that raffinose gene family is stimulated in cold, heat and anaerobic conditions. Abiotic stress can regulate the expression of raffinose gene family through binding to these cis-acting elements in upstream of VvGolS and VvRfS genes [4]
Keywords:
Abiotic stress; Carbohydrates; Galactinol synthase; Raffinose synthase
Status : Paper Accepted (Poster Presentation)