1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Differences between hg-19 and hg-38 in transcriptomic analysis of cumulus cells in PCOS patient
Paper ID : 1122-ICB10
Authors:
mohammad yahya vahidi mehrjardi *1, Mohammadhosein Fakherbaheri2, Kimia Monshizadeh2
1Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2Student Research Committee, Shaid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:
Absteract : Understanding of oocyte maturation process is prominent in infertility malady’s such as Polycystic ovary syndrome(PCOS). PCOS patients need to be assisted reproduction technologies (ARTs) like IVF for fertilization. So this is a prerequisite to identify oocyte with reproductive power. In this study we analyzed cumulus cells which are around oocytes, they are appropriate choice due to their directive relationship with oocyte and separating of them is a non-invasive method to check oocyte maturation. We analyzed 5 transcriptomic profile cumulus samples from PCOS patients ( 2 sample were for GV oocyte and 3 samples were for MII oocyte ) with RNA-Seq metod with hg-19 and hg-38. This study showed us 1775 DEG with hg-19 and 1403 with hg-38 because of this were various significant pathways and gene ontologies. Based on the different gene expressions of hg19 and hg38, Real- time PCR should be applied to confirm expression differences .
Keywords:
PCOS , cumulus cell , hg-19 , hg-38 , transcriptomic analysis
Status : Paper Accepted (Poster Presentation)