1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
A computationally optimized broadly reactive antigen analysis of tick midgut surface protein Bm86 in vaccine development against Rhipicephalus microplus and Rhipicephalus annulatus
Paper ID : 1142-ICB10
Authors:
Mohammad Mehdi Ranjbar1, Gholamreza Karimi1, Nayebali Ahmadi2, Sajjad Yazdansetad *2
1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:
Background: The tick-borne diseases cause significant economic losses in animals and humans. The tick midgut surface protein Bm86 of Ripsephalus spp. is considered as an immunoreactive antigen for vaccine candidate against cattle fever ticks. In this bioinformatics report, we focused on the computationally optimized broadly reactive antigen (COBRA) tool to decipher the tick midgut protein Bm86 for vaccine strategy in the fight against Rhipicephalus species.
Materials and Methods: In this bioinformatics analysis, the consensus sequences of R. microplus and R. annulatus were retrieved, aligned with the GenBank data using the BLASTN algorithm and Sequencher v4.8 program, and finally edited. The phylogenetic trees were built using the maximum likelihood method. Bm86-based vaccine using the COBRA method was designed according to the taxa grouping and the center-of-tree (COT) sequences. The protein superimposition algorithms and molecular modeling analysis were also carried out.
Results: The hypervariable regions were identified in the amino acid residues 177-181, 270-276, and 351-352, respectively in both R. microplus and R. annulatus sequences. Six sequences were selected to anticipate the evolutionary vaccine and 12 sequences were used to realign and obtain the consensus sequences to do COBRA. R. annulatus sequences were in sister branches with more similarity to each other compared to Bm86 protein sequences in R. microplus except ADQ19687. The sequences AJE29931, AJE29932, ATW75472, ATW5476, ADM86722, and ACZ55133 were selected for vaccine designing according to the ancestral center-of-tree (COT).
Conclusion: Anti-tick vaccines using the COBRA approach could be a more cost-effective alternative with a broader spectrum compared to the commonly used recombinant vaccines.
Keywords:
Rhipicephalus; Bm86; Phylogeny; Vaccine; COBRA
Status : Paper Accepted (Poster Presentation)