1st International and 10th National Iranian Conference on Bioinformatics

Bioinformatics study of miR-301a as a blood-borne biomarker for detection of breast cancer

Paper ID : 1149-ICB10

Authors:

Reza Ghanbari, Ghazal Ziarizi, Mohsen Hosseini, Mohammad Niazi, Mansoureh Azadeh *

Zist Fanavari Novin biotechnology institute

Abstract:

Introduction
MicroRNAs are small non-coding molecules, which play a regulatory role in post-transcriptional gene expression. In this study, using bioinformatics databases, expression levels, target genes, cellular location and genetic pathways of miR-301a were investigated as a blood-borne biomarker for detection of breast cancer.
Methods
Focusing on bioinformatics aspects, miRbase and GEO databases were used to determine chromosomal profile, target genes and validation of miR-301a as a blood-borne biomarker. The cellular location of miR-301a and its expression levels in different organs, especially the breast in a healthy and tumorous state were evaluated in the GeneCards and GEPIA2 database. Finally, the DAVID database was used for genetic pathways analysis.
Results and Discussion
MiR-301a gene is located on chromosome 17q22. The miR-301a is overexpressed in breast cancer and plays a role in the post-transcriptional pathway of approximately 2760 genes which could lead to a variety of diseases by mutations in these genes. Among these genes, mutations in 151 of them can cause breast cancer. MiR-301a can be widely found in whole blood because it was identified to be transported out of the cells after transcription.
Conclusion
Current study reveals that miR-301a plays a role in the post-transcriptional pathway of a large number of genes. It is also highly expressed in breast cancer as a blood-borne biomarker. As a result, miR-301a has a great potential for clinical diagnosis of breast cancer.

Keywords:

Blood-borne biomarker, Breast cancer, MiR-301a

Status : Paper Accepted (Poster Presentation)