1st International and 10th National Iranian Conference on Bioinformatics

Bioinformatic analysis of differentially expressed genes in Ulcerative Colitis

Paper ID : 1164-ICB10

Authors:

Mahsa Ekhtiar, pegah Javid, Mansoureh Azadeh *

Zist Fanavari Novin biotechnology institute

Abstract:

Ulcerative colitis (UC) is an idiopathic inflammatory colon disease characterized by widespread friability and superficial erosions of the colonic wall, as well as bleeding [1]. It is the most prevalent type of inflammatory bowel disease in the world [2]. The aim of our study was to determine the potential biomarkers as targets for treatment of UC. Thus, the gene expression profile was analyzed through GEO datasets based on GSE11223 to search out the top differentially expressed genes where analyzed in DAVID database. MicroRNAs and SNPs involved in UC were studied through miRdSNP. The LncRNADisease database revealed the long noncoding RNAs. According to GEO analysis, IL1b, FCGR3, IRAK3, and TIMP1 were considered as Up-regulated genes. The miRNAs and SNP involved in UC were hsa-miR-578, hsa-miR-122, hsa-miR-224, and rs315951 respectively. In addition, the lncRNAs H19 and BC012900 were implicated in UC as well. The Genes’ biological mechanisms and pathways were enriched in interleukins and cytokines, linked to different pathways such as inflammatory responses and TNF signaling pathway. The results reveal that the occurance of rs315951 at the binding site of hsa-miR-578, at 965 nucleotides upstream of hsa-miR-224, or at 625 nucleotides upstream of hsa-miR-122 on 3’UTR of IL1RN gene, downregulate the expression of IL1RN and may cause UC disease. It means that the expression of IL1RN gene inhibits the activity of interleukin-1 by binding to IL1R1 receptor. H19 overexpression reduced Vitamin D receptor (VDR) expression significantly. VDR signaling has been considered to play an important role in the regulation of inflammation and has a vital role in intestinal epithelial barrier. Inflammatory cytokines promote BC012900, and it cause apoptosis in intestinal epithelial cells. It is concluded the mentioned genes, miRs, and SNP could raise our understanding of UC pathogenesis and IL1RN may be used as a potential therapeutic purpose for UC patients.

Keywords:

UC; differentially expressed genes; miRNA, SNP, lncRNA; Inflammation

Status : Paper Accepted (Poster Presentation)