1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
The gene expression patterns of stromal cells in prostate tissue during prostate cancer
Paper ID : 1182-ICB10
Authors:
Maryam Rahimi Tesiye *1, Mona Zaersabet2, Amir Charkaneh2, Zahra Abrishami Kia3, Elnaz Khorasanian1
1Faculty of Life Science and Biotechnology, Shahid Beheshti university, Tehran, Iran
2Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
3Department of exercise physiology, Faculty of physical education and sport sciences, University of Mazandaran, Babolsar, Mazandaran Province, IRAN
Abstract:
Introduction: The development of stromal cells in prostatic glands revealed that these cells not only nourish and support the secretory epithelium of the glands, but also contribute to the morphogenesis, its hormonal metabolism and secretory function. It has also been exhibited that they are involved in the onset and development of cancer by producing reactive stroma [1,2]. Accordingly, this study aims to analyze the gene expression patterns of stromal cells in prostate gland during prostate cancer in comparison to normal tissue.
Methods: The information related to normal prostate tissue was obtained by GEO database with GSE 26910 and the stromal cells’ data was provided by GSE 35373. Four sets of data were gained for each group and the platform used for both was [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array. Data analysis was performed by R software. After entering the commands related to directory setting, showing microarray images, drawing histogram, data were normalized. Afterwards, heatmap commands were typed in order to observe the gene expression patterns.
Results and discussion: The results of data analysis and heatmap illustration showed that there was a significant difference between the gene expression patterns of stroma and normal cells which indicates the importance of these cells in tumorigenesis. Given the substantial difference in the gene expression patterns of stromal cells and normal tissue as well as the complexity of prostate cancer treatment, it seems that more attention to these cells could be an important therapeutic goal.
Keywords:
Keywords: stromal cells, prostate cancer, gene expression, prostate gland, R software
Status : Paper Accepted (Poster Presentation)