1st International and 10th National Iranian Conference on Bioinformatics
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Bioinformatics Study on Slc34a2 Gene in Lung Cancer Tumorigenesis
Paper ID : 1189-ICB10
Authors:
Atena Zadeh rokni1, Pegah Javid2, Mansoureh Azadeh *1, Fatemeh Razavi1
1Zist Fanavari Novin Biotechnology Department, State Technical and vocational Training Organization, Isfahan, Iran
2Zist Fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran
Abstract:
Abstract
A novel paradigm in tumor biology suggests that Non-Small Cell Lung Cancer (NSCLC) growth is driven by lung cancer stem cell-like cells (LCSCs) [1]. SLC34A2, a member of the solute carrier gene family, encodes NaPi2b (the type II Na/Pi co-transporter) [2]. NaPi2b is a multi-transmembrane sodium-dependent phosphate transporter responsible for transcellular inorganic phosphate absorption, maintenance of phosphate homeostasis, and tumorigenesis [3]. The biological function of SLC34A2 and its underlying mechanisms in NSCLC has remained unclear [3]. In this study, the SLC34A2 gene was the target for bioinformatics findings. For this purpose, single nucleotide polymorphism (SNP) and microRNA (miRNA) associated with SLC34A2 were found in the GeneCards and NCBI databases. LncRNADisease (version 2.0) database helped to analyze the data to find a long non-coding RNA (lncRNA) related to same miRNA. The analysis showed that the occurance of the SNP:rs5996397 can lead the cells to lung cancer and affect the involved miRNA-650 and lncRNA-MEG3 in this process. The rs5996397 occures at the seed match of miRNA-650 on 3’UTR of SLC34A2 gene. According to findings from the GEPIA2 database, the SLC34A2 gene is highly expressed in lung cancer. Through the process of this disease, the disease-associated SNP: rs5996397 is located on the mRNA and causes the disease. When miRNA-650 binds to 3’UTR of SLC34A2 mRNA, it increases gene expression and intensifies the tumorigenesis of LCSCs. When lncRNA-MEG3, which is a tumor suppressor, binds to miRNA-650 and suppresses SLC34A2, it silences miRNA-650 and reduces pathogenicity. based on different aspects of this pathway, it is inferred that the interaction of lnc-MEG3 with rs5996397 could be useful biomarkers for the diagnosis of lung cancer. In conclution, the miRNA-650 prove that SLC34A2 is an effective gene in lung cancer outbreak and its tumorigenesis.
Keywords:
Key words: NSCLC; miRNA-650; rs5996397; lnc-MEG3; SLC34A2.
Status : Paper Accepted (Poster Presentation)