1st International and 10th National Iranian Conference on Bioinformatics

Transcriptome profiling analysis of Suaeda salsa shoots under salinity stress

Paper ID : 1190-ICB10

Authors:

Shima Jamalirad *

Abstract:

Salinity is one of the consequential abiotic stresses that significantly impact agricultural yield worldwide. euhalophyte Suaeda salsa is a model plant that may help identify salt tolerance molecular mechanisms to develop salt-resistant crops. In the present study, high-throughput RNA sequencing was conducted after the Suaeda salsa seedlings were exposed to 0 mM, 200 mM, 400 mM, and 800 mM NaCl for 30 days. The comparative analysis of transcripts identified 610, 1893, and 1691 genes significantly expressed at 200mM, 400mM, and 800 mM NaCl, compared to control, respectively. Gene Ontology (GO) and MapMan analyses revealed that the differentially expressed genes were related to solute transport and nutrient uptake, protein synthesis, modification, hemostasis, transcriptional regulation, and Phytohormones action. Moreover, we identified multiple genes associated with different transcription factor families, including 143 DEGs, which may regulate salt stress-response in S. salsa. Finally, the DEGs in the salt response pathway were verified by Quantitative real-time PCR (qRT-PCR).

Keywords:

Salinity, Euhalophyte, Suaeda salsa, High-throughput RNA sequencing

Status : Paper Accepted (Oral Presentation)