1st International and 10th National Iranian Conference on Bioinformatics

Bioinformatics study of level expression of miR-212 as a biomarker of Glioblastoma

Paper ID : 1200-ICB10

Authors:

Ali Attaripour Isfahani, Mohammad Sina Pirmoradian, Elnaz Nasiriyan, Zahra Rafiezadeh, Mansoureh Azadeh *

Zist Fanavari Novin biotechnology institute

Abstract:

Introduction
MicroRNAs are small non-coding nucleic acids (around 20 nucleotides) that are able to post-transcriptionally regulate gene expression by binding to complementary sequences of target messenger RNA. In this study, miR-212 expression levels, target genes and genetic pathways in glioblastoma were investigated using a bioinformatics database.
Methods
This bioinformatic study was performed by several main databases, such as " miRdSNP, mirbase, GeneCards and DAVID". At first from mirbase, the chromosomal profile of this miRNA was obtained. The position of miR-212 on the human chromosome and complete information about that, was identified In GeneCards. Finally the DAVID database was used for genetic pathways evaluation.
Results and Discission
MiR-212 gene is located on chromosome 17p13.3. miR-212 is overexpressed in cancers of the central nervous system and is found in various parts of the cell, particularly in the extracellular space and plasma membrane. miR-212 plays a role in the post-transcriptional pathway of 2950 genes of cellular sections. In addition, of the 2562 identified miR-212 gene targets, which with influence on these genes could result in a variety of diseases, it can act as a tumor suppressor in glioblastoma by effecting on 53 genes. It was also found that miR-212 by Suppression of SGK3 gene as an important tumor-promoting gene is directly involved in the suppression of glioblastoma. Based on these results, miR-212 inhibits glioblastoma cell proliferation.
Conclusion
The current study shows that, increased expression of miR-212 in brain cells as a tumor suppressor, can indicate the presence of cerebral glioblastoma, which may also lead to a rapid diagnosis of this chronic disease. Especially, since miR-212 can also be detected in serum and plasma, which are much more readily obtainable than tissues, it attracts increased clinical attention as a biomarker in glioblastoma.

Keywords:

Key words: Glioblastoma, miR-212, Tumor suppressor, Tumor cell biomarker

Status : Paper Accepted (Poster Presentation)