1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Genome-wide transcriptome analysis reveals many alternative splicing events in peanut roots (Arachis hypogaea L.) under drought stress conditions
Paper ID : 1211-ICB10
Authors:
Zahra Ghorbanzadeh1, Feba Jacob2, Mohammad Reza Ghaffari1, Rasmieh Hamid *3
1Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREO)
2Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, India
3Department of Plant Breeding, Cotton Research Institutes of Iran (CRII), Agricultural Researches, Education and Extension Organization, Gorgan, Iran
Abstract:
Alternative splicing (AS) is a posttranscriptional regulatory mechanism that promotes proteome diversity. AS is often observed in the context of changing environmental conditions [1, 2]. However, it is unclear whether or not AS plays a role in plant response to drought stress [3, 4]. In this work, we conducted a genome-wide survey of AS events in peanut (Arachis hypogaea) roots grown under normal and drought conditions. Under normal conditions, drought stress, and post-drought recovery, we found 234, 456, and 415 AS events, respectively, that were substantially differentially spliced. Alternative skipped exons and splice sites were the most common types of AS. These drought-treated root samples produced 856 genes with significant AS change. Enrichment analysis revealed that AS modulation of binding activity is critical for peanut root response to drought. Specifically, genes encoding the splicing regulatory components of the spliceosome pathway and the mRNA surveillance pathway were enriched in the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Genes related to the splicing regulatory factor of peanut root also responded to drought stress and were alternatively spliced. In summary, our results suggest that genes with specific AS patterns could be used to improve plant adaptation to drought stress. These discoveries would open new avenues for enhancing plant stress resistance as the role and mechanism of AS in the process of abiotic stress are further explored.
Keywords:
Alternative splicing, Drought stress, Peanut, Root
Status : Paper Accepted (Poster Presentation)