1st International and 10th National Iranian Conference on Bioinformatics

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Evaluation of ZmNAC10 gene sequence and protein structure of this gene to evaluate salinity stress resistance in plants

Paper ID : 1213-ICB10

Authors:

Iman Yousefi Javan *, Masoud Alipanah

گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه تربت حیدریه، تربت حیدریه، ایران

Abstract:

Abstract
Corn is one of the most important cereals of American origin after wheat and has the largest agricultural lands in the world.This plant contains nutrients and can be a good source of fiber. Due to the value of this plant, recognizing the genes involved in the path of resistance to stress is one of the most important issues of this plant. Irrigation method, environmental factors and medical stresses on it. To better understand and understand the molecular mechanism for resistance to stress, the gene and expression of ZmNAC10 were specifically studied. NACs are one of the largest family of plant-specific transcription factors (TFs) and play a key role in growth and stress responses. The sequence of this gene is highly similar to the sequence of receptor kinases (high-affinity cell surface receptors) [1]. In this study, ZmNAC10 gene was studied using bioinformatics sources and primers were designed to amplify this gene. Then, its cDNA was made by reverse transcriptase enzyme and used as a template for ZmNAC10 gene amplification in PCR reaction. ZmNAC10 gene expression was lowest in stems and roots and highest in leaves [2]. During the PCR reaction, a 561 bp fragment of cDNA and a 1440 bp fragment of genomic DNA were amplified [3]. This gene encodes a protein of 187 amino acids that has a region with a kinase sequence [4]. By analyzing the molecular structure of the transcribed protein, the isoelectric point and the molecular weight were obtained, and also based on the homologous modeling, its spatial shape showed that this protein has a regular structure [5].

Keywords:

Keywords: Molecular mechanism, Resistance to stresses, Kinases Receptor, Homologous modeling

Status : Paper Accepted (Poster Presentation)