1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Liver tissue transcriptome data analysis for identification of key miRNAs in sheep (Ovis aries) with different dites
Paper ID : 1217-ICB10
Authors:
Sajed Sarabandi1, Farinaz Behfarjam2, Mostafa Rafiepour *2
1Department of Veterinary, Faculty of Medical Sciences, Islamic Azad University of Karaj, Karaj, Iran
2Department of Genetics, Faculty of Science, Danesh Alborz University, Qazvin, Iran
Abstract:
Background: Today, genetic engineering methods used to change the genome in a way to produce more products like milk [1]. Understanding regulatory mechanisms and the identification of small RNAs involved in metabolism is one of the most challenging problems in genetic science. Therefore, the aim of the present study was to identify some regulator genes in Ovis aries with different diets using bioinformatics analysis.
Materials and Methods: In this research, 13 samples of Illumina-sequence transcriptome data for sheep (Ovis aries) livers with different diets (high energy group n=3, oil supplement group n=3, light grazing n=2, over-grazing n=2 and control samples n=3) were collected. Firstly, quality control of data were done with FastQC program. Then, the reads aligned to the reference genome (ARS-UI_Ramb_v2.0) with STAR software. Finally, to identify the differentially expressed miRNAs, data were analyzed using FeatureCounts and DESeq2 programs, respectively.
Result: Our results showed several differentially expressed miRNAs in each group (high energy group n=13, oil supplement group n=11, light grazing n=10 and overgrazing n=10). For example, in oil diet, miRNA221 and miRNA29B significantly upregulated (p<0.05) that related to glucose metabolism [2]. Also, the result showed expression of miRNA30 increased in oil supplement groups which has a key role in lipid metabolism [3]. In the high energy and overgrazing groups, miRNA143 was significantly downregulated (p<0.05). Studies showed that this microRNA is an important regulator in glucose metabolism [4]. In addition to, the resulted showed that the expression of mir16b, mir30b, and mir199a significantly decreased (p<0.05) in all groups. Studies indicated that these miRNAs have the important roles in glucose methbolism and immune system response [4, 5].

Conclusion: In this study, our results indicated that different diets are determinant in regulating of key pathways and mechanisms like lipid metabolism and miRNAs can be candidate targets for purposeful nutrition.
Keywords:
Metabolism, miRNA, Ovis Aries, Gens expression, Bioinformatic
Status : Paper Accepted (Poster Presentation)