1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
In silico transcriptome analysis of drought and salt involved responsiveness genes in Brassica napus
Paper ID : 1222-ICB10
Authors:
mohadese Dousti *, Leila Mazhary, Zarrin Minuchehr, Tahmineh Lohrasebi, Khadijeh Razavi
National Institute of Genetic Engineering and Biotechnology
Abstract:
Canola (Brassica sp.) as the most important oily seed product in the world is affected largely by salinity and drought stresses due to planting in arid and semi-arid regions [1, 2]. So, studying about impressive genes in salt/drought stresses in canola would be helpful to improve tolerant species. In this study genes that involved in salt and drought stresses in Brassica napus were investigated from integrating gene expression omnibus (GEO) and sequence read archive (SRA) databases in different time line since 2021. Then results were analyzed by web based software GEO2R and GALAXY for detect differentially expressed genes (DEGs). DEGs in short, mid and long-term of salinity and drought stresses were obtained from extensive meta-analysis (RRA method). Subsequently ontology of acquired robustDEGs were done by blast2go. By constructing PPI network with Cytoscape software, hub genes in each time line were identified. Among all 34 A. thaliana hub genes, HAI2 and DREB1B were selected to validate with real time qPCR in tolerant (Okapi) and sensitive (RGS) cultivars of canola. The hub known and novel genes that identified through our meta-analysis, provide insight to understanding the molecular response to salinity/drought stresses and engineering abiotic stress tolerance in canola.
Keywords:
Brassica napus; salinity; drought; meta analysis; candidate genes
Status : Paper Accepted (Oral Presentation)