1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
hsa-miR-8081 is a novel key regulatory RNA in the thyroid cancer development by regulation of HMGA2 in the transcriptional misregulation in cancer signaling pathway: high-throughput data analysis of microarray experiment
Paper ID : 1248-ICB10
Authors:
Mina Dehghani Samani *, Khatereh Firouzi-Farsani
گروه ژنتیک،دانشکده علوم پایه،دانشگاه شهرکرد،شهرکرد،ایران
Abstract:
Gene interaction networks, in which genes activate and repress the transcription of other genes, are responsible for much of cellular life's complexity, and their failure can be fatal to an organism. As a result, systems biology has long sought to comprehend these networks [1]. This research performed an integrated computational study to demonstrate a novel gene regulatory network and select a significant microRNA-mRNA interaction axis in the thyroid cancer samples.
Gene expression analysis of thyroid cancer samples was performed by microarray data analysis of the GSE33630 microarray dataset. Data analysis of microarray samples was performed by affy [2] and limma [3] statistical packages in R Studio (4.0.2) environment. microRNA – mRNA interaction analysis was performed by miRWalk [4]. Pathway enrichment and Gene Ontology analysis were performed by Enrichr online software [5].
Microarray data analysis revealed that HMGA2 has a significantly high expression (logFC: 5.225, adjusted. P. Value < 0.0001) in patients with thyroid cancer. Also, miRWalk analysis revealed that hsa-miR-8081 regulates the expression level of HMGA2 in the 3UTR region (Score: 1). The HMGA2 is a key regulatory gene in the transcriptional misregulation in the cancer signaling pathway. This protein has a significant role in the histone-serine phosphorylation (GO:0035404) in the nuclear chromosome (GO:0000228) and nuclear lumen (GO:0031981).
Based on this integrated high-throughput and RNA interaction analysis, for the first time, we demonstrated that hsa-miR-8081 could be a critical regulatory RNA in the thyroid cancer patients, by regulation of HMGA2 – a potential biomarker and oncogene of thyroid cancer - mRNA level and affecting the histone-serine phosphorylation in the nuclear lumen. It is highly recommended that a luciferase assay experiment validates the mentioned RNA interaction to achieve more accurate information about the precise role of mentioned microRNA in thyroid cancer development.
Keywords:
Systems biology; Big biological data; Microarray; HMGA2; microRNA
Status : Paper Accepted (Poster Presentation)