1st International and 10th National Iranian Conference on Bioinformatics

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SERPINB2 Overexpression As a Potential Contributor to Development of Multiple Myeloma And Its Predictive CeRNA Network

Paper ID : 1249-ICB10

Authors:

Ali Barani, Mohammad Rezaei, Mansoureh Azadeh *

Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran

Abstract:

Abstract
Multiple myeloma (MM) is a type of bone marrow cancer in which monoclonal plasma cells undergo rapid growth, resulting in overexpression of immunoglobins[1]. The exceptional growth of information and advances in technology in biomedical fields has been overwhelming for labratorary scientists over the last decade. Therefore, research has been moving from being strictly conducted in a real life lab environment to a ‘virtual lab’ environment where management and analysis of data are done[2].
Initially, gene expression data of MM patients (GSE146649) was obtained from the NCBI Gene Expression Omnibus (GEO) and then analyzed by GEO2R to find differentially expressed genes (DEGs). GENT2[3] database was then used to reinforce the possibility of correlation between the selected gene and bone marrow cancer. Through UniProt[4] and GeneCards[5], gene ontology information and biological pathway involvement were understood. Furthermore, miRWalk[6], TargetScan[7] and miRDB[8] were utilized to find significant miRNA-mRNA interactions in the 3’UTR region. Additionally, the selected miRNA was searched in LncBase v.2[9] to find strong interactions with lncRNAs and construct a predictive ceRNA network.
Through analysis of the GEO dataset, a gene named SERPINB2 was found to be considerably upregulated (|logFC| = 4.246 , adj. P value =4.10e-04) in MM samples. The product of this gene is a protein called plasminogen activator inhibitor 2 (PAI-2) which mainly inhibits urokinase-type plasminogen activator in fibrinolysis pathway but it can also protect the cell from apoptosis by interacting with Proteasome subunit beta type-1 (PSMβ1). Analysis of possible miRNA-mRNA interactions revealed hsa-miR-182-5p as a significant interactor to SERPINB2 mRNA. This miRNA was then searched in LncBase v.2 (Bone marrow) and MIR663AHG, FAM106A and LINC00963 had the strongest interactions.
In conclusion, SERPINB2 is overexpressed in MM and forms a possible ceRNA network among hsa-miR-182-5p, MIR663AHG, FAM106A and LINC00963.

Keywords:

Multiple Myeloma; SERPINB2; Cancer; CeRNA

Status : Paper Accepted (Poster Presentation)