1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Dysregulation of LINC01985 lncRNA leads to the abnormal exocytosis by low expression of MMRN1 in the thyroid cancer patients: an integrated computational biology approach
Paper ID : 1258-ICB10
Authors:
خاطره فیروزی فارسانی *, مینا دهقانی سامانی
گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران
Abstract:
Genomic characterization and evolutionary histories of both primary and metastatic cancer development have been made possible by high-throughput and multi-omics technologies. These tools, which give insight into cancer cells' genome, transcriptome, metabolome, and proteome, confirm our grasp of systems biology-level approaches to cancer [1]. This study performed a high-throughput microarray data analysis to find novel regulatory genes in the thyroid cancer samples, using a systems biology approach. Furthermore, the RNA-interaction and functional enrichment analysis was performed to demonstrate the precise regulatory mechanism of the coding and non-coding RNAs found in the experiment. The GSE33630 microarray dataset was analyzed by R Studio (4.0.2). Normalization of raw data was performed by affy package [2]. Differential expression (DE) and statistical analyses were performed by limma package [3]. RNA interaction and functional enrichment analyses (Pathway and Gene Ontology (GO) ) was performed by Enrichr online software [4]. MMRN1 has a significantly low expression in the thyroid cancer patients, compared to control (logFC: -4.205, adj. P. Value < 0.0001). LINC00477 and LINC01985 lncRNAs have a significant co-expression with the NMRN1 mRNA (adjusted p-value: 0.005). Based on literature mining, LINC00477 isoforms can have a significant role in developing gastric cancer (by down-regulation) [5]. In this study, we demonstrate the possible regulatory role of the two mentioned lncRNAs in developing thyroid cancer for the first time by disturbance of the regulation of exocytosis (GO:0045055) in the secretory granule lumen (GO:0034774). LINC00477 and LINC01985 lncRNAs are the two novel non-coding RNAs that can regulate the expression of MMRN1. Dysregulation of the two mentioned RNAs can lead to the abnormality in the expression of MMRN1 and disturb the regular exocytosis mechanism in the secretory granule lumen in thyroid cancer patients.
Keywords:
Systems biology; High-throughput data; Microarray; MMRN1; RNA interaction
Status : Paper Accepted (Poster Presentation)