1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Investigation of Novel Regulators of the COPD Development Based on Systems Biology Approaches: A WGCNA Study
Paper ID : 1278-ICB10
Authors:
Hojat Borna, B. Fatemeh Nobakht M. Gh., Yazdan Hasani Nourian, Masoud Arabfard *
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Abstract:
Background: Chronic Obstructive Pulmonary Disease (COPD) is a well-known chronic disease, which is the third worldwide disease-related leading cause of death (1). A complex combination of factors over a long time prepares conditions for emergence of this progressing disease. Understanding genetic basics and behavior of the major actors of the disease including immune systems Polymorphonuclear Cells (PMNCs), sheds light on turning points of COPD development (2).
Method: To achieve the goal, different transcriptomics containing databases were surveyed to find proper COPD containing microarray data. Chosen dataset (GSE42057) (3) was submitted to analyze with WGCNA and Limma, in order to find co-expressed genes as well as relationship between modules and clinical traits and possible differentially expressed genes, respectively.
Results: Data analysis with Limma was unable to present any possible Differentially Expressed Gene (DEG). However, expression network analysis revealed prominent hub genes as well as strong relationships between some identified modules and Eigengenes with clinical variables (4). It is revealed that two highly altered modules containing mostly inflammatory and remodeling pathways are highly related with BMI and golden stages of COPD.
Conclusion: It is interesting that cellular integrity control genes such as tubulin proteins(TUBA1A), aquaporins (AQP1), peroxisome proliferator-activated receptor (PPAR), and Nitric Oxide Synthase 2(NOS2) are also discovered to be significantly involved in the pathogenesis of the process.
Keywords:
COPD, WGCNA, Module, PMNC
Status : Paper Accepted (Poster Presentation)