1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Design, In silico analysis, expression and evaluation of hybrid and bivalent forms of scFvs against Neisseria meningitides factor H binding protein (fHbp)
Paper ID : 1294-ICB10
Authors:
fatemeh yarian *1, mona Salahshoor Dahr2, Abbas Alibakhshi3, Shahrzad Ahangarzadeh4, maryam ghane5, marjan hkajavi6
1Department of medical biotechnology, schol of advanced technology in medicine, fasa unoversity of medical sciences
2دانشگاه ازاد اسلامی شهریار
3دانشگاه علوم پزشکی همدان
4Isfahan University of Medical Sciences
5دانشگاه ازاد اسلامی-اسلامشهر
6دانشگاه ازاد اسلامی
Abstract:
Meningococcal disease is an acute disease caused by the bacterium Neisseria meningitidis. Humans are the only natural hosts of this bacterium. It has several pathogenic factors, among which the factor H binding protein (fHbp) plays a vital role in the survival of the pathogen in the host [1]. The single-chain variable fragment antibody (scFv) have been shown to potentially be well used in therapeutic and diagnostic applications against this factor [2]. With the aim of stronger effect, in the present study, two scFvs with anti-fHbp potency [3] were constructed in hybrid format and docked to fHbp antigen. An anti-fHbp bivalent format of the scFvs was also constructed. Both formats were obtained by expression into pET28a vector and purified by affinity chromatography. For determination of affinity of the hybrid and bivalent formats, ELISA method was used. According to bioinformatics results, one of the hybrid formats with a suitable structure was selected. The affinity of selected hybrid format was 7.6 × 10-9 M and the affinity for bivalent format was 3.77 × 10-9 M. The higher affinity of hybrid format indicates that development of scFv hybrid formats seems to be a better solution than bivalent format.
Keywords:
,In silico analysis, Hybrid scFv; bivalent scFv; Neisseria meningitides; factor H binding protein
Status : Paper Accepted (Poster Presentation)