1st International and 10th National Iranian Conference on Bioinformatics

Polymorphisms effects on structure and function of OeACP2 and OeACP3 proteins

Paper ID : 1301-ICB10

Authors:

Maryam Beizaei Nejad *, Tayebeh Jadidi, Khadijeh Razavi, Vahid Shariati, Seyed Mahdi Hosseini Mazinani, Zarrin Minuchehr

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Abstract:

Olive oil has fundamental positive influences on human health and prevent cardiovascular diseases [1]. Fatty acid biosynthesis (FAS) pathways produce and store lipids in mesocarp of olive (Olea europaea L.) [2]. An important cofactor of fatty acid biosynthesis is acyl carrier protein (ACP) that acts as a shuttle. It makes covalent binds with fatty acyl intermediates and moves them from previous enzyme to next enzyme of the pathway [3]. So mutations in ACPs may affect and disrupt fatty acid biosynthesis and oil producing [4]. ACP has three loci in plants that each of them has two alleles. OeACP2 and OeACP3 have more crucial role and largely found in olive fruit and leave [5]. Hence, we extracted DNA from two Iranian olive cultivars, Shenge and Mari, with low and high quality oil respectively. Then whole genome next generation sequencing (NGS) was done and mutations in these genes were found. We identified single nucleotide polymorphisms (SNPs), insertions and deletions (InDels) in DNA sequences and predicted tertiary structure of proteins with online servers such as I-TASSER and Robetta by homology modeling and ab initio methods. Finally we found some effective polymorphisms that seem to impact on structure and function of acyl carrier proteins.

Keywords:

olive oil; FAS; ACP; polymorphism

Status : Paper Accepted (Poster Presentation)