1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Integrated Bioinformatics Analysis of Key Genes in Hepatitis B Virus-Associated Hepatocellular Carcinoma
Paper ID : 1308-ICB10
Authors:
Adel Rezvani Sichani1, Ziba Rezvani Sichani1, Elham Naderi Semiromi1, Mohammad Rezaei2, Mansoureh Azadeh *1
1Zist Fanavari Novin biotechnology institute
2Zist Fanavari Novin Biotechnology institute
Abstract:
The e majority of primary liver cancers in adults worldwide are hepatocellular carcinomas (HCCs, or hepatomas).Hepatocellular carcinoma (HCC) is one of the most lethal cancers globally.The purpose of this study is to find the key genes in Hepatitis B Virus-Associated Hepatocellular Carcinoma Gene expression profiles of GSE55092, GSE94660, GSE121248 from the GEO
database were obtained and analyzed.There were 120 HCC samples and 138 nontumour tissue samples Functional assessment of DEGs was performed by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG).enrichment analyses showed that these DEGs were mainly enriched in cell division and DNA replication biological processes, nucleoplasm and microtubule cellular components, protein binding molecular functions, and cell cycle and DNA replication pathways.Key genes were selected by the protein-protein interaction (PPI) network and further validated by GSE25599 clinical data. Through protein– protein interaction analysis,14 Key gene DEGs were indicated,including CDKN3,CCNA2,PBK,CCNB2,CCNB1,MAD2L1,FEN1,CDC20,TPX2,BUB1B,TOP2A,RRM2,ECT2,CDK1. that closely related to the of hepatitis B. In addition, in comparison with normal tissues, the expression levels of TOP2A, MAD2L1,CDC20, and CCNB2 were higher in HBVrelated HCC. In conclusion, our results suggest that CDC20 and TOP2A might serve as a key gene for prognosis and as a therapeutic target for HBVassociated HCC
Keywords:
Hepatocellular Carcinoma, Hepatitis B, Key gene, Bioinformatics Analysis
Status : Paper Accepted (Poster Presentation)