1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Immunoinformatics approach for designing poly-epitope based vaccines
Paper ID : 1325-ICB10
Authors:
Elham Ghafouri *1, Hossein Khanahmad2
1گروه ژنتیک و بیولوژی مولکولی- دانشکده پزشکی دانشگاه علوم پزشکی اصفهان-اصفهان- ایران
2گروه ژنتیک و بیولوژی مولکولی- دانشکده پزشکی- دانشگاه علوم پزشکی اصفهان- اصفهان- ایران
Abstract:
Abstract
The primary purpose within all the immunizations is the ability of the vaccine to induce a
robust immune response in a faster mode than the pathogen itself. Designing the best vaccine
in the fastest time is very important. Reverse vaccinology employs bioinformatics to find
proteins and epitopes that indicate antigenicity. Some of the advantages of this approach are
reduced cost and time of vaccine development and facilitating antigen identification
selection. This method does not require the cultivation of risky microorganisms, allowing to
study non-cultivate microorganisms and screening of all microorganism proteins from
genome sequences, making them biologically safe. Additionally, their selectivity allows
accurate activation of immune responses.
Methods: We will highlight the immune-informatics and computational approach technique
known as reverse vaccinology to predict the potential epitope for designing poly epitope
vaccines, including the T cell and B cell epitopes. Each epitope's antigenicity, toxicity, and
allergenicity are checked; then, the selected epitopes are joined together via different linkers.
Various physical-chemical properties of the poly-epitopes vaccine, including molecular
weight (MW), isoelectric point (IP), stability index, half-life in vitro and in vivo, aliphatic
index, and grand average of hydropathicity (GRAVY), are estimated in silico. The threedimensional structure of the mentioned vaccine will be subjected to molecular docking
studies with MHC-I and MHC-II molecules.
Results: In the final, we selected the non-toxic and non-allergic epitopes with a high
antigenicity score. These epitopes are linked together and indicate suitable properties.
Conclusion: The proposed vaccine needs to be validated clinically to ensure its safety and
immunogenic profile.
Keywords:
Keywords: Reverse vaccinology; Immuno-informatics; Poly epitopes; T-and B-cell epitopes; Vaccine design
Status : Paper Accepted (Poster Presentation)