1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
A bioinformatics analysis of exosomal microRNAs related to colorectal cancer: An approach for identifying diagnostic biomarkers
Paper ID : 1350-ICB10
Authors:
Ali khalafizadeh *1, Seyedeh Donya Hashemizadegan1, majid sadeghizadeh2, sadegh babashah1
1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Demarteman of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:
Background:
Colorectal cancer (CRC) has shown a remarkable increase in the annual global incidence rate among prevalent kinds of gastrointestinal malignancies [1]. Exosomes have recently been recognized as an important player in CRC pathogenesis. They are secreted nanoparticles that are present in both normal and pathophysiological conditions in all body fluids and are also effective in cell-cell communication. microRNAs (miRNAs), as the most important cargo in exosomes, are potential cancer biomarkers that can be useful in diagnosing and prognosis. To find a potent diagnostic biomarker, we checked the EVmiRNAs, a database of miRNA profiling in extracellular vesicles, and re-analyzed the expression profiles of specific exosomal miRNAs in CRC that were present in 11 samples.
Methods:
The expression of exomiRs was studied using the EVmiRNA database. This database provided 535 small RNA sequencing samples of EVs from 11 sources / diseases and constructed the EVmiRNA database (http://bioinfo.life.hust.edu.cn/EVmiRNA) to show the miRNA expression profiles. We selected 10 miRNAs (hsa-miR-10a-5p, hsa-miR-192-5p, hsa-miR-191-5p, hsa-miR-92a-3p, hsa-miR-21-5p, hsa-miR-148a-3p, hsa-miR-182-5p, hsa-let-7a-5p, hsa-miR-215, hsa-miR-27b-3p) that have most expression in CRC from EVmiRNA database and used Diana miRpath V.2 algorithm (http://diana.imis.athena-innovation.gr/DianaTools) to make a heatmap.
Results:
We found that among these selected exosomal miRNAs that have highest expression in CRC, hsa-miR-21-5p and hsa-let-7a-5p have high expression in pathways in cancer and P53 signaling pathway by Diana miRpath V.2 algorithm. We demonstrated that these miRNAs have specific roles in these pathways in CRC and we found that our selected miRNAs have high expression in these pathways in CRC.
Conclusion:
We established that hsa-miR-21-5p and hsa-let-7a-5p as exosomal miRNAs have the potential of targeting crucial pathways in CRC that can be chosen as a target for CRC.
Keywords:
Keywords: colorectal cancer, microRNA, EVmiRNA database, Diana miRpath V.2
Status : Paper Accepted (Poster Presentation)