1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Identification of key transcription factors and microRNAs associated with drought stress in rice: a systems biology approach
Paper ID : 1365-ICB10
Authors:
Ali Asghar ali Dehghan *1, Ali Moghadam2, Yaser Biniaz3, ali M dehghan2, Akbar M Karegar1
1bajgah-agriculture shiraz
2Institute of Biotechnology, Shiraz University, Shiraz, Iran
3Plant Virology Research Center, Shiraz University, Shiraz, Iran
Abstract:
Drought is one of the major environmental risks that decreases the successful production of crops, especially rice, in the world, which can occur at any time during the growing season. Hence, one of the main challenges in agriculture is to produce more food with less water. Therefore, study of molecular mechanisms in order to characterization of key gene networks responsive to this stress is more considered. For this purpose, we retrieved the transcriptome RNA seq data from the European Bioinformatics Institute (EBI) database. Data quality analysis and final analysis were performed using CLC Genomics Workbench 20.0. The reference genome as well as the rice annotation were downloaded from the NCBI. Adjusted P-values (FDR < 0.001) were considered significant and, differentially expressed genes (DEGs) were used for further analysis. Analysis of GO enrichment for DEGs showed that transcripts were more involved in cellular processes such as cellular process and localization. In addition, In the category of cellular components, DEGs were enriched for cellular anatomical presence and intracellular anatomical structure. In the category of molecular function most genes were referred to ion membrane transmitter activity, transmembrane transporter activity of molecular entity. Based on the results of Kyoto Encyclopedia of Genes and Genomes analysis, it was concluded that endocytosis and mRNA surveillance pathways, these two had a greater role in the plant's response to drought stress. In microRNA analysis, miR156, miR160, miR171, and miR172 showed the highest expression in drought stress conditions. In the analysis of transcription factors, it was shown that the bHLH family had the highest expression in both down-regulated and up-regulated genes in response to drought stress. Also, transcription factors bZIP, WRKY, MYB, C2H2, which play a key role in drought resistance and stress, had the highest increase in expression.
Keywords:
Key words: Abiotic stress, microRNA, System biology, Drought
Status : Paper Accepted (Poster Presentation)