1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Bioinformatic analysis of long non-coding RNA ZFAS1 that is involved in colorectal cancer via indirect correlation
Paper ID : 1375-ICB10
Authors:
seyedeh donya hashemizadegan manshadi *1, Ali Khalafizadeh1, majid sadeghizadeh2, sadegh babashah1
1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Demarteman of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:
Background and aim:
Colorectal cancer (CRC) is the second and third most frequently diagnosed cancer in women and men, respectively, with an increasing global incidence rate [1]. Many researchers have recently focused on long non-coding RNAs (lncRNAs) because of their abnormal expression patterns in CRC and their potential function in carcinogenesis as oncogenes or tumor suppressors. LncRNAs are non-protein coding RNA molecules with a length of more than 200 nucleotides [2].
Methods:
To find a potent diagnostic biomarker, we determined RNA-seq expression profiles of 50 lncRNAs, selected between tumor and non-tumor colorectal tissues, and identified a signature of lncRNAs differentially expressed in CRC patients. Based on the fold-change (p<0/05), we selected 10 lncRNAs and then used research articles that focused on them, finally selecting Zinc Finger Antisense 1(ZFAS1). Furthermore, we used the non-code database (http://www.noncode.org/), and LNCipedia (http://www.lncipedia.org) and finally, we found an indirect correlation between ZFAS1 and the p53 signaling pathway.
Results:
ZFAS1 has been increased in CRC and plays a role in proliferation, invasion, and EMT. ZFAS1 operates as an oncogene in CRC by deregulating p53 indirectly, causing cell cycle advancement and apoptosis inhibition. In CRC, overexpression of ZFAS1 resulted in decreased p53 protein expression, increased proliferation and cell cycle arrest, and inhibited apoptosis.
Conclusion:
We established that ZFAS1 plays a role in the p53 network via deregulation of p53 and inhibition of apoptosis with indirect correlation, indicating that ZFAS1 participates in the p53‐mediated apoptosis pathway in CRC.
Keywords:
Keywords: colorectal cancer, lncRNA, ZFAS1, p53 signaling pathway
Status : Paper Accepted (Poster Presentation)