1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Genome-wide profiling of DNA/RNA hybrids (R-loops) in psoriasis disease
Paper ID : 1398-ICB10
Authors:
Leila Kianmehr *1, hassan rajabi-maham1, Minoo Rassoulzadegan2
11 Animal Sciences and Biotechnology Department, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University,Tehran, Iran
2Université de Nice, INSERM-CNRS, France
Abstract:
DNA/RNA hybrids (R-loops) are RNA-mediated epigenetic element involved in the molecular mechanism of human diseases. R-loops are three-stranded structure made up a DNA/RNA hybrid and the displaced single-stranded DNA. Recently proposed independent-antibody method of our group leads to detect these structures in different type of cells started from germ cells to currently somatic cells. One physiological instance of such structure present at our chromosomes ends (telomeres) as a lncRNA named TERRA (TElomeric Repeat-containing RNA; constitutive of hexameric repeats (TTAGGG/CCCTAA)n. Using TERRA as a privilege model system to study R-loops helps us to understand DNA/RNA hybrids are accumulated at the telomeres of different chromosomes in psoriasis patient samples (lesional and non-lesioanl skin samples vs. healthy controls) as previously observed in germ cells. It is reported that short telomeres are associated with higher TERRA levels in aging research. TERRA/DNA hybrids opens new startegies to study psoriasis genome instability. Distribution of different non-coding RNA besides TERRA throughout the genome of psoriasis-derived skin cells revealed the unresolved DNA/RNA hybrids may facilitate skin lesions formation and may play an important role in epigenome shaping.
Keywords:
DNA/RNA hybrids, R-loops, epigenetics, RNA-seq, psoriasis, TERRA
Status : Paper Accepted (Poster Presentation)