1st International and 10th National Iranian Conference on Bioinformatics
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Selection of blood biomarkers to diagnose therapeutic resistance in people with epilepsy
Paper ID : 1400-ICB10
Authors:
Foroozan Fatahinia *1, سعید لطیفی نوید2, کامران قائدی3
14. Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.
2دانشیار گروه آموزشی زیست شناسی
3استاد تمام سلولی مولکولی،، دانشگاه اصفهان
Abstract:
Epilepsy is a common brain disease affecting 70 million people worldwide. Antiepileptic drugs dramatically prevent seizures in patients, but about one-third of patients are resistant to these drugs. Therefore, finding blood biomarkers helps to diagnose and treat patients. Research shows that mutations in genes encoding ion channels play a role in epilepsy.LncRNAs play an important role in the central nervous system, with dysfunction and expression changes associated with epilepsy.
Method: Initially, Array express and GEO databases were examined to identify and express gene changes in this disease. Finally, the GSE71058 study, which is a type of RNA sequencing technology, was selected. According to the type of data, R software and related packages were used for analysis. After evaluation and changes in gene expression, a list of genes and LncRNAs that had more than double and significant expression changes (pvalue <0.05) were selected among the study groups. Because these ion channels play an important role in epilepsy, the list of genes related to ion channels was extracted using the HGNGO database. Also, to identify lncRNAs related to ion channel genes, the correlation between them was evaluated using the Pearson correlation coefficient. At this stage, lncRNAs with a correlation coefficient greater than +0.7 or less - 0.7 and a significant level of pvalue <0.05 were selected.

Results: According to the analyzes performed on the GSE71058 data, the hcn1, hcn2, hvcn1 genes, which are related to ion channels, and the LncRNAs of FOXN3-AS1 and SLC26A4-AS1 have more than double the expression changes and have become.

Conclusion: Considering the results of data analysis and changes in the expression of ion channel genes and related LncRNAs and their importance in causing epilepsy, it is suggested that changes in gene expression be studied in a detailed laboratory study.
Keywords:
epilepsy, ions channel, biomarker
Status : Paper Accepted (Poster Presentation)