1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Bioinformatics analysis of changes FNDC5 gene expression in skeletal muscle of whey protein-treated mice
Paper ID : 1410-ICB10
Authors:
Alieh Abdolrezaie *1, Hashem Nayeri1, Ali Mohammad Ahadi2
1Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
2Department of Genetic, School of Science, Shahr-e-kord University, Shahr-e-Kord, Iran
Abstract:
Background: Irisin production is a metabolic pathway that results from the poroteolytic breakdown of the FNDC5 protein, which is secreted from skeletal muscle as an exercise hormone during exercise [1]. Considering that food compounds can affect metabolic pathways, gene expression and protein production [2], the aim of this study was to investigate the results of the effect of whey protein on the expression of some genes, production of dependent proteins and the study of thermogenesis in skeletal muscle.
Materials and Methods: In this study, 20 male mice were divided into four groups: placebo, supplementation treatment, exercise and simultaneous supplementation and exercise treatment. Supplementation was performed with whey protein. Then muscle tissue samples were taken and the expression of FNDC5 gene in muscle was evaluated. Finally, we were investigated the correlation of FNDC5 gene expression with genes involved in skeletal muscle thermogenesis, protein interaction and metabolic pathways with string databases and cytoscape software.
Results: The results showed that whey protein supplementation had a positive effect on FNDC5 gene expression and bioinformatics analysis confirmed the correlation between irisin levels, gene expression and thermogenesis. also found that UCP1 and PPARa are genes in the process of thermogenesis in skeletal muscle that are associated with FNDC5 / Irisin in this metabolic pathway.
Conclusion: Protein compounds with their structure and components have a great impact on the expression of genes and metabolism in the body [3]. Therefore, it was found that whey protein increases the level of irisin by activating molecular signaling pathways and increasing gene expression. Today, the use of protein is a strategy to treat obesity and improve athletic performance [4], and protein supplements seem to be the most suitable combination for achieving high doses of Irisin and thermogenesis.
Keywords:
Key words: FNDC5, Bioinformatics, Gene expression, Irisin, Whey protein
Status : Paper Accepted (Poster Presentation)