1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Investigation of renal tubular cells gene expression during acute kidney injury induced by Cisplatin
Paper ID : 1450-ICB10
Authors:
Marjan Nejati1, MohammadReza Esmaeili2, sara taleahmad *2, Reza Moghadasali3
16th Floor, No.10, Roodaki Street, Farajollahi alley, Tehran, Iran
2Royan Institute
3مدیر گروه بیماری‌های کلیوی پژوهشگاه رویان
Abstract:
Acute kidney injury (AKI) is defined by a sudden loss of excretory kidney function. (1) AKI is part of a range of conditions summarized as acute kidney diseases and disorders (AKD), in which slow deterioration of kidney function or persistent kidney dysfunction is associated with an irreversible loss of kidney cells and nephrons, which can lead to chronic kidney disease (CKD). (2, 3, 4) As a consequence, finding signaling pathways related to the proximal tubule injury by bioinformatics approaches is essential. In this study we focused on gene expression profiling in damaged Tubular cells and investigated signaling pathways associated with this matter. At first, we selected suitable studies from GEO database (GSE85957). This dataset consists of three groups of injured PTCs with four difference times (D3, D5, D8 and D26) and analyzed with GEO2R tool. Then uploaded the up and down regulated genes to VENNY version 2.1.0 tool and selected processed in common genes between up/down regulated groups to examine the signaling pathways in the DAVID database and the KEGG library then loaded the genes involved in biological process, cell component and molecular function. Finally, we used STRING database to select protein interaction.
Results showed that 127 genes up regulated and 128 genes were down regulated. Among those up regulated genes most of them were expressed in p53 signaling pathway, Ribosome, TNF signaling pathway, HTLV-I infection in other hand metabolic pathways, Citrate cycle (TCA cycle) and Carbon metabolism were observed in down regulated genes. Mdm2، Cdkn1a، Egr1، Fos، Rps7، Atf3 genes were up regulated and the results also obtained that Adk, Pdha1 ،Sdha، Sdhd، Uqcrc2 were down regulated. By this analysis and results we observed the genes that identified in tubular cells damages that highly related to ribosome, metabolic pathways.
Keywords:
Acute kidney disease, Proximal tubule cell, Microarray analysis, gene expression profile
Status : Paper Accepted (Poster Presentation)