1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Analysis of targeted base ITS sequences in the genus Astragalus using Hill Climbing Algorithm
Paper ID : 1454-ICB10
Authors:
Negar Karimi *1, حجت اله سعیدی2
15th Ramazan St. 16 alley, number 21, 3rd floor
2گروه زیست شناسی گیاهی و جانوری، دانشکده علوم و فناوری های زیستی، دانشگاه اصفهان، اصفهان، ایران
Abstract:
The genus Astragalus is an important taxon with recognized center/centers of diversification and origination in Iran. different species of the genus relating to old and new world occurs in Iran. So, this region has been of great attentions considering phylogenetic studies of the genus. In the present study 30 accessions of different species of the genus were sequenced targeting ITS1 region and 32 seqneced datasets were added from NCBI data base, making a complete representative of 10 phylogenetically close sections of the genus in Iran. Forward and reverse contigs were alighned and used for further analysis. The Hill Climbing algorithm was chosen as the best model representing phylogenetic relationships, testing by GTR+I+G method and, selected through AKAIKE INFORMATION CRITERTION test, by the generation of 2,000,000 generations. The validity of the number of generations was checked with Tracer software. The resulting standard deviation was estimated to be 0.022. The outgrouping taxa were defined and the resulting tree was plotted using FigTree software. The Consistency Index (CI) was calculated for the tree as; CI= 0.391. The results indicated that different species were grouped based on their similarity and dissimilarity and grouped mainly based on their evolutionary priorities defining two specific clades relating to old and new world respectively. Among them 5 species A. aegobromus, A. coluteocarpus, A. siliquosus, A. zerdanus and A. hamosus, all belonging to the old world, were grouped apart. The rest of the species belonging to 2 sections of Hymenostegis and Anthylloidei were grouped in a specific clade, being a representative of the new world taxa occurring in Iran. The results indicated that although the genus Astragalus has been experiencing different geographical and ecological conditions in Iran, the species occurring in Iran follow thier ancestral relationships and possess a potential germplasm to be used with conservation porposus.
Keywords:
Fabaceae, Phylogeny, Bayasian method, ITS, Iran
Status : Paper Accepted (Poster Presentation)