1st International and 10th National Iranian Conference on Bioinformatics

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Hsa-miR-24 is a novel and critical regulatory biomarker of LBL by regulation of cell growth signaling pathway: integrated microarray and bioinformatics analyses

Paper ID : 1465-ICB10

Authors:

Saba Hojjati, shadi omidghaemi, Mansoureh Azadeh *

Zist Fanavari Novin biotechnology institute Strategic & Modern Technologies Skill training center , Technical and Vocational Organization, Hezar Jerib street, Isfahan, Iran

Abstract:

Abstract
Lymphoblastic leukemias lymphomas (B-All / LBL) account for 2% of lymphoid neoplasms of precursor T-Cells or lymphoblasts. The incidence appears to be rising in both children and adults. B-Cells acute lymphoproliferative disease manifest as pure leukemia (B-All) in 80% of cases, isolated extramedullary presentations frequently have marrow involvement at diagnosis that may be morphologically biomarker may require detection by high-resolution flow cytometry. In recent decades, miRNAs and lncRNAs have been studied and are considered impactful biomarkers in cancer. Therefore, in this bioinformatic approach, the goal was to spot and determine a biological network of genes, miRNAs, and lncRNAs, which have a notable influence on progression LBL [1-3]. Microarray data analysis of the LBL gene expression profile was performed by GEO2R online software. GSE29986 was analyzed in this study. Using miRWalk [4] and miRdSNP [5], microRNA – mRNA interaction analysis was performed. The common microRNAs were selected by venny 2.1 [6] online software. Pathway enrichment and mRNA interaction analyses were performed by DAVID [7]. Single nucleotide polymorphism analysis was performed by miRNASNP v3 [8]. Based on microarray analysis by GEO2R, PDGFRB has a significant dysregulation in the LBL samples compared to control (adj. p. value < 0.0001). DAVID database revealed that PDGFRB and the related mRNAs, EBF1, LPAR1, WNT6, and FN1, are the essential genes in the cell energy and growth signaling pathway. Also, hsa-miR-24 is the commonly interacted microRNAs with all of mentioned five genes, and its binding affinity is correlated to the rs246387. In conclusion, hsa-miR-24 regulates the cell growth pathway and LBL development by suppressing the expression of mentioned genes, especially in the rs246378 region of the PDGFRB gene.

Keywords:

Keywords: (B-ALL), LBL, PDGFRB, ra246387, has-miR-24

Status : Paper Accepted (Poster Presentation)