1st International and 10th National Iranian Conference on Bioinformatics
Home Accepted Papers
Integrated Bioinformatics Analysis of Genes Associated with Lung Cancer Pathway
Paper ID : 1478-ICB10
Authors:
Zahra Mosayebi Dorcheh1, Pegah Javid2, Mansoureh Azadeh *3
1Zist fanavari Novin biotechnology institute
2Zist Fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran
3Zist Fanavari Novin biotechnology institute Strategic & Modern Technologies Skill training center , Technical and Vocational Organization, Hezar Jerib street, Isfahan, Iran
Abstract:
Lung Cancer (LC) is a disorder that influences lung tissue cells [1]. LC is generated while bizarre cells are divided in an out of control manner to shape a tumor withinside the lungs [2]. The remedy technique relies upon at the form of most cancers in addition to the patients’ trendy health [3]. Mortality from lung most cancers may be drastically decreased with early detection of the disorder [4]. In this study, the bioinformatics analysis was conducted to find more information about the involved markers and pathway through the disease. For this purpose, NCBI, miRNASNP, miRdSNP, miRBase, miRWalk, and DAVID databases were surveyed. In this regard, miRNASNP and miRdSNP databases were used to identify related microRNAs (miRs) and single Nucleotide Polymorphisms (SNPs) in LC. Studies at NCBI indicated that three of the most active genes involved in LC, are TPM3 gene (Located on chromosome 1), ITGB3 gene (Located on chromosome 17) and CYP1B1 gene (Located on chromosome 2). Studies in miRdsNP database showed that in the process of LC disease, the conversion of T to C base through dSNP rs1051370 in nucleotide 2179 could affect the binding of miRNA has-miR-1 to associated 3’UTR of TPM3 gene on chromosome 1 and change its function. In addition, the occurrence of has-miR-1 sequence on 3’UTR of TPM3 gene on chromosome 1 would change its function. Furthermore, has-miR-1, has-let-7a and has-miR-27b could be the most important microRNAs associated with these genes. The dSNP rs2317676 occurs on nucleotide 712 at the junction of has-let-7a to 3’UTR of ITGB3 gene and converts the alleles A to allele G. In the process of LC, the has-miR-27b binds to 3’UTR of CYP1B1 gene on chromosome 2 and the dSNP rs9341266 accrues in nucleotide 694 where C is converted to T. It is concluded that since changes in sequences of genes mediated by dSNP can change their function, it could be a potential marker through disease diagnosis.
Keywords:
miRNA; cancer; dSNP; miRdSNP; fatal diseas
Status : Paper Accepted (Poster Presentation)